NOTO Protein (AA 1-251) (His tag)
custom-made
NOTO
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- 抗原 See all NOTO products
- NOTO (Notochord Homeobox (NOTO))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-251
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This NOTO protein is labelled with His tag.
- 原理
- Custom-made recombinant NOTO Protein expressed in mammalian cells.
- 序列
- MPSPRPRGSP PPAPSGSRVR PPRSGRSPAP RSPTGPNTPR APGRFESPFS VEAILARPDP CAPAASQPSG SACVHPAFWT AASLCATGGL PWACPTSWLP AYLSVGFYPV PGPRVAPVCG LLGFGVTGLE LAHCSGLWAF PDWAPTEDLQ DTERQQKRVR TMFNLEQLEE LEKVFAKQHN LVGKKRAQLA ARLKLTENQV RVWFQNRRVK YQKQQKLRAA VTSAEAASLD EPSSSSIASI QSDDAESGVD G Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
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Notochord Homeobox (NOTO) (AA 1-240) protein (His tag)
custom-made NOTO 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- NOTO (Notochord Homeobox (NOTO))
- 别名
- NOTO (NOTO 产品)
- 背景
- Homeobox protein notochord,FUNCTION: Transcription regulator acting downstream of both FOXA2 and Brachyury (T) during notochord development. Required for node morphogenesis. Is essential for cilia formation in the posterior notochord (PNC) and for left-right patterning, acts upstream of FOXJ1 and RFX3 in this process and is required for the expression of various components important for axonemal assembly and function. Plays a role in regulating axial versus paraxial cell fate. Activates the transcription of ciliary proteins C11orf97 homolog, FAM183B and SPACA9 in the embryonic ventral node (By similarity). {ECO:0000250|UniProtKB:Q5TIS6}.
- 分子量
- 27.0 kDa
- UniProt
- A8MTQ0
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