Cytochrome b Protein (AA 1-380) (His tag)
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- 抗原 See all Cytochrome b (MT-CYB) 蛋白
- Cytochrome b (MT-CYB) (Mitochondrially Encoded Cytochrome B (MT-CYB))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-380
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Cytochrome b protein is labelled with His tag.
- 原理
- Custom-made recombinant MT-CYB Protein expressed in mammalian cells.
- 序列
- MTPMRKTNPL MKLINHSFID LPTPSNISAW WNFGSLLGAC LILQITTGLF LAMHYSPDAS TAFSSIAHIT RDVNYGWIIR YLHANGASMF FICLFLHIGR GLYYGSFLYS ETWNIGIILL LATMATAFMG YVLPWGQMSF WGATVITNLL SAIPYIGTDL VQWIWGGYSV DSPTLTRFFT FHFILPFIIA ALATLHLLFL HETGSNNPLG ITSHSDKITF HPYYTIKDAL GLLLFLLSLM TLTLFSPDLL GDPDNYTLAN PLNTPPHIKP EWYFLFAYTI LRSVPNKLGG VLALLLSILI LAMIPILHMS KQQSMMFRPL SQSLYWLLAA DLLILTWIGG QPVSYPFTII GQVASVLYFT TILILMPTIS LIENKMLKWA Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
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Mitochondrially Encoded Cytochrome B (MT-CYB) (AA 1-381) protein (His tag)
custom-made MT-CYB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitochondrially Encoded Cytochrome B (MT-CYB) (AA 1-98), (full length) protein (GST tag)MT-CYB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- Cytochrome b (MT-CYB) (Mitochondrially Encoded Cytochrome B (MT-CYB))
- 别名
- MT-CYB (MT-CYB 产品)
- 背景
- Cytochrome b (Complex III subunit 3) (Complex III subunit III) (Cytochrome b-c1 complex subunit 3) (Ubiquinol-cytochrome-c reductase complex cytochrome b subunit),FUNCTION: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis. {ECO:0000250|UniProtKB:P00157}.
- 分子量
- 42.7 kDa
- UniProt
- P00156
- 途径
- Proton Transport
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