DRD4 蛋白
This Synthetic Nanodisc DRD4 protein is expressed in Mammalian Cells.
Quick Overview for DRD4 蛋白 (ABIN7538217)
抗原
See all DRD4 蛋白
DRD4
(Dopamine Receptor D4 (DRD4))
蛋白类型
Synthetic Nanodisc
宿主
人
资源
Mammalian Cells
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原理
- Human DRD4 full length protein-synthetic nanodisc
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产品特性
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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Dopamine Receptor D4 (DRD4) (AA 1-387) protein (His tag)
custom-made DRD4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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说明
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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有效期
- 12 months
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- DRD4 (Dopamine Receptor D4 (DRD4))
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别名
- DRD4
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背景
- This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats, the sequence shown contains four repeats. [provided by RefSeq, Jul 2008]
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分子量
- The human full length DRD4 protein has a MW of 43.9kDa
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UniProt
- P21917
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途径
- cAMP Metabolic Process, Synaptic Membrane, Proton Transport, Photoperiodism, Negative Regulation of Transporter Activity
抗原
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