Osteoactivin Protein (GPNMB) (His tag)
Quick Overview for Osteoactivin Protein (GPNMB) (His tag) (ABIN7535384)
抗原
See all Osteoactivin (GPNMB) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This Osteoactivin protein is labelled with His tag.
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原理
- Active Recombinant Human Osteoactivin/GPNMB Protein
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序列
- MECLYYFLGF LLLAARLPLD AAKRFHDVLG NERPSAYMRE HNQLNGWSSD ENDWNEKLYP VWKRGDMRWK NSWKGGRVQA VLTSDSPALV GSNITFAVNL IFPRCQKEDA NGNIVYEKNC RNEAGLSADP YVYNWTAWSE DSDGENGTGQ SHHNVFPDGK PFPHHPGWRR WNFIYVFHTL GQYFQKLGRC SVRVSVNTAN VTLGPQLMEV TVYRRHGRAY VPIAQVKDVY VVTDQIPVFV TMFQKNDRNS SDETFLKDLP IMFDVLIHDP SHFLNYSTIN YKWSFGDNTG LFVSTNHTVN HTYVLNGTFS LNLTVKAAAP GPCPPPPPPP RPSKPTPSLG PAGDNPLELS RIPDENCQIN RYGHFQATIT IVEGILEVNI IQMTDVLMPV PWPESSLIDF VVTCQGSIPT EVCTIISDPT CEITQNTVCS PVDVDEMCLL TVRRTFNGSG TYCVNLTLGD DTSLALTSTL ISVP
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特异性
- Met1-Pro474
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过滤
- 0.22 μm filtered
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内毒素水平
- <0.1EU/μg
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Human GPNMB Protein at 1 μg/mL (100 μL/well) can bind GPNMB Rabbit pAb with a linear range of 0.976-116.5 ng/mL.
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Osteoactivin (GPNMB) (AA 23-502) protein (His tag)
GPNMB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Osteoactivin (GPNMB) (AA 23-486) protein (His tag)GPNMB 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Osteoactivin (GPNMB) (AA 23-496) protein (His tag)GPNMB 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Osteoactivin (GPNMB) (AA 22-474) protein (His tag)GPNMB 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
Osteoactivin (GPNMB) (AA 22-474) protein (His tag)GPNMB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
Osteoactivin (GPNMB) (AA 1-572) protein (GST tag)GPNMB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Osteoactivin (GPNMB) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GPNMB 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- Osteoactivin (GPNMB)
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别名
- Osteoactivin/GPNMB
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背景
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Description: Transmembrane glycoprotein NMB (GPNMB) is also known as Transmembrane glycoprotein HGFIN, DC-HIL and Osteoactivin (OA), which belongs to the PMEL/NMB family. GPNMB contains one PKD domain.GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization and functions as a negative regulator of inflammation in macrophages. Osteoactivin is expressed at high levels in normal and inflammatory liver macrophages suggesting a significant role in acute liver injury. The early-phase upregulation of Osteoactivin expression in the tubular epithelium in response to renal injury might play a role in triggering renal interstitial fibrosis via activation of matrix metalloproteinase expression and collagen remodeling in rats. Osteoactivin is a protein that is expressed in aggressive human breast cancers and is capable of promoting breast cancer metastasis to bone.
Name: GPNMB,HGFIN,NMB
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基因ID
- 10457
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UniProt
- Q14956-2
抗原
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