Nuclease 蛋白
宿主: Serratia marcescens
宿主: Yeast (Pichia pastoris)
Recombinant
> 95 % by SDS-PAGE.
Active
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- 抗原 See all Nuclease products
- Nuclease
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 宿主
- Serratia marcescens
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资源
- Yeast (Pichia pastoris)
- 原理
- Active Recombinant Serratia marcescens Nuclease
- 序列
- DTLESIDNCA VGCPTGGSSN VSIVRHAYTL NNNSTTKFAN WVAYHITKDT PASGKTRNWK TDPALNPADT LAPADYTGAN AALKVDRGHQ APLASLAGVS DWESLNYLSN ITPQKSDLNQ GAWARLEDQE RKLIDRADIS SVYTVTGPLY ERDMGKLPGT QKAHTIPSAY WKVIFINNSP AVNHYAAFLF DQNTPKGADF CQFRVTVDEI EKRTGLIIWA GLPDDVQASL KSKPGVLPEL MGCKN
- 特异性
- Asp22-Asn266
- 纯度
- > 95 % by SDS-PAGE.
- 内毒素水平
- <0.1EU/μg
- Biological Activity Comment
- 1.One unit (U) is defined as the amount of enzyme required to change the absorption value of A260 by 1.0 (equivalent to complete digestion of 37 μg of salmon essence DNA into oligonucleotides) in 30 min at 37°C, pH 8.0 reaction conditions. The specific activity of Serratia marcescens nuclease is > 1000 U/μL.|2.Recombinant Serratia marcescens Nuclease (0.1U) can effectively degrade 5 μg plasmid DNA at 37°C for 30 min. The reaction buffer is: 10 mM MgCl2, 0.1 mg/mL BSA, 50 mM Tris-HCl, pH8.5.
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Solution in 50 % glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.
- 储存条件
- -20 °C
- 储存方法
- This product is stable at -15°C ~ -25°C for up to 2 year from the date of receipt.|For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
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- 抗原
- Nuclease
- Abstract
- Nuclease 产品
- 背景
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Description: Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA.
Name: Endonuclease, Nuclease, nucA, nuc
- 基因ID
- 66715254
- UniProt
- P13717
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