IgG2a 蛋白
This Recombinant IgG2a protein is expressed in HEK-293 Cells.
Quick Overview for IgG2a 蛋白 (ABIN7536588)
抗原
IgG2a
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
HEK-293 Cells
纯度
> 97 % by SDS-PAGE.
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原理
- Active Recombinant Human IgG2A Protein
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序列
- ERKCCVECPP CPAPPVAGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVQFNWYV DGVEVHNAKT KPREEQFNST FRVVSVLTVV HQDWLNGKEY KCKVSNKGLP APIEKTISKT KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDISV EWESNGQPEN NYKTTPPMLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
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特异性
- Glu99-Lys326
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过滤
- 0.22 μm filtered
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内毒素水平
- <0.1EU/μg
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA.Immobilized Human CD32a at 2 μg/mL (100 μL/well) can bind Human IgG2 with a linear range of 1.2-338 ng/mL.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- IgG2a
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物质类
- Antibody
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背景
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Description: Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens
Name: IGHG2,Immunoglobulin heavy constant gamma 2,IgG2
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基因ID
- 3501
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UniProt
- P01859
抗原
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