Endoglin Protein (ENG) (His tag)
Quick Overview for Endoglin Protein (ENG) (His tag) (ABIN7535480)
抗原
See all Endoglin (ENG) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This Endoglin protein is labelled with His tag.
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原理
- Active Recombinant Human Endoglin/CD105 Protein
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序列
- ETVHCDLQPV GPERGEVTYT TSQVSKGCVA QAPNAILEVH VLFLEFPTGP SQLELTLQAS KQNGTWPREV LLVLSVNSSV FLHLQALGIP LHLAYNSSLV TFQEPPGVNT TELPSFPKTQ ILEWAAERGP ITSAAELNDP QSILLRLGQA QGSLSFCMLE ASQDMGRTLE WRPRTPALVR GCHLEGVAGH KEAHILRVLP GHSAGPRTVT VKVELSCAPG DLDAVLILQG PPYVSWLIDA NHNMQIWTTG EYSFKIFPEK NIRGFKLPDT PQGLLGEARM LNASIVASFV ELPLASIVSL HASSCGGRLQ TSPAPIQTTP PKDTCSPELL MSLIQTKCAD DAMTLVLKKE LVAHLKCTIT GLTFWDPSCE AEDRGDKFVL RSAYSSCGMQ VSASMISNEA VVNILSSSSP QRKKVHCLNM DSLSFQLGLY LSPHFLQASN TIEPGQQSFV QVRVSPSVSE FLLQLDSCHL DLGPEGGTVE LIQGRAAKGN CVSLLSPSPE GDPRFSFLLH FYTVPIPKTG TLSCTVALRP KTGSQDQEVH RTVFMRLNII SPDLSGCTSK G
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特异性
- Glu26-Gly586
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过滤
- 0.22 μm filtered
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内毒素水平
- <0.1EU/μg
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Biological Activity Comment
- 1.Measured by its binding ability in a functional ELISA. Immobilized Human CD105 at 2 μg/mL (100 μL/well) can bind Human ACVR2B with a linear range of 0.01-1.35 μg/mL.|2.Measured by its binding ability in a functional ELISA. Immobilized Human CD105 at 2 μg/mL (100 μL/well) can bind Human TGFBR2 with a linear range of 0.01-1.72 μg/mL.
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Endoglin (ENG) (AA 26-586) protein (His-Avi Tag)
ENG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Endoglin (ENG) (AA 1-658) protein (GST tag)ENG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Endoglin (ENG) (AA 394-581) protein (His-IF2DI Tag)ENG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Endoglin (ENG) (AA 27-658) protein (GST tag)ENG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Endoglin (ENG) (AA 27-581) (Active) protein (His tag)ENG 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS, AcA Active
Endoglin (ENG) (AA 26-586) protein (His tag)ENG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85% by SDS-PAGE SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- Endoglin (ENG)
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别名
- Endoglin/CD105
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背景
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Description: Endoglin, also known as CD105, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type II receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.
Name: END,HHT1,ORW1,CD105,ENG,endoglin
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基因ID
- 2022
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UniProt
- P17813
抗原
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