CA2 蛋白
Quick Overview for CA2 蛋白 (ABIN7533772)
抗原
See all CA2 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- Active Recombinant Human Carbonic anhydrase 2 Protein
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序列
- SHHWGYGKHN GPEHWHKDFP IAKGERQSPV DIDTHTAKYD PSLKPLSVSY DQATSLRILN NGHAFNVEFD DSQDKAVLKG GPLDGTYRLI QFHFHWGSLD GQGSEHTVDK KKYAAELHLV HWNTKYGDFG KAVQQPDGLA VLGIFLKVGS AKPGLQKVVD VLDSIKTKGK SADFTNFDPR GLLPESLDYW TYPGSLTTPP LLECVTWIVL KEPISVSSEQ VLKFRKLNFN GEGEPEELMV DNWRPAQPLK NRQIKASFK
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特异性
- Ser2-Lys260
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过滤
- 0.22 μm filtered
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内毒素水平
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its esterase activity. The specific activity is >840 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
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Carbonic Anhydrase II (CA2) (AA 1-260) protein (GST tag)
CA2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase II (CA2) (AA 1-260) proteinCA2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Carbonic Anhydrase II (CA2) (Active) protein (His tag)CA2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 96 % as determined by SDS-PAGE Active
Carbonic Anhydrase II (CA2) (AA 1-260) (Active) proteinCA2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS Active
Carbonic Anhydrase II (CA2) (AA 1-260) protein (His tag)CA2 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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缓冲液
- Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
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储存条件
- -20 °C,-80 °C
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储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- CA2 (Carbonic Anhydrase II (CA2))
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别名
- Carbonic anhydrase 2
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背景
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Description: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification.
Name: CA2, CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282, carbonic anhydrase 2,CA-II,CAC,CAII,Car2,HEL-76,HEL-S-282
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基因ID
- 760
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UniProt
- P00918
抗原
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