alpha Fetoprotein Protein (His tag)
Quick Overview for alpha Fetoprotein Protein (His tag) (ABIN7519667)
抗原
See all alpha Fetoprotein (AFP) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This alpha Fetoprotein protein is labelled with His tag.
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原理
- Active Recombinant Human Alpha-fetoprotein/AFP Protein
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序列
- RTLHRNEYGI ASILDSYQCT AEISLADLAT IFFAQFVQEA TYKEVSKMVK DALTAIEKPT GDEQSSGCLE NQLPAFLEEL CHEKEILEKY GHSDCCSQSE EGRHNCFLAH KKPTPASIPL FQVPEPVTSC EAYEEDRETF MNKFIYEIAR RHPFLYAPTI LLWAARYDKI IPSCCKAENA VECFQTKAAT VTKELRESSL LNQHACAVMK NFGTRTFQAI TVTKLSQKFT KVNFTEIQKL VLDVAHVHEH CCRGDVLDCL QDGEKIMSYI CSQQDTLSNK ITECCKLTTL ERGQCIIHAE NDEKPEGLSP NLNRFLGDRD FNQFSSGEKN IFLASFVHEY SRRHPQLAVS VILRVAKGYQ ELLEKCFQTE NPLECQDKGE EELQKYIQES QALAKRSCGL FQKLGEYYLQ NAFLVAYTKK APQLTSSELM AITRKMAATA ATCCQLSEDK LLACGEGAAD IIIGHLCIRH EMTPVNPGVG QCCTSSYANR RPCFSSLVVD ETYVPPAFSD DKFIFHKDLC QAQGVALQTM KQEFLINLVK QKPQITEEQL EAVIADFSGL LEKCCQGQEQ EVCFAEEGQK LISKTRAALG V
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特异性
- Arg19-Val609
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过滤
- 0.22 μm filtered
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内毒素水平
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Human AFP at 2 μg/mL (100 μL/well) can bind AFP Mouse mAb with a linear range of 0.061-5.431 ng/mL.
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alpha-Fetoprotein (AFP) (AA 19-605) protein (His tag)
AFP 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
alpha-Fetoprotein (AFP) (AA 19-609) protein (His tag)AFP 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
alpha-Fetoprotein (AFP) (AA 19-609) protein (His tag)AFP 宿主: 犬 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
alpha-Fetoprotein (AFP) (AA 19-609) protein (His tag)AFP 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
alpha-Fetoprotein (AFP) (AA 19-609) protein (Fc Tag)AFP 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
alpha-Fetoprotein (AFP) (AA 500-609) protein (GST tag)AFP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
alpha-Fetoprotein (AFP) (AA 1-609) protein (GST tag)AFP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
alpha-Fetoprotein (AFP) (AA 21-605) protein (His tag)AFP 宿主: 小鼠 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
alpha-Fetoprotein (AFP) (AA 19-605) protein (His tag)AFP 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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别名
- Alpha-fetoprotein/AFP
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背景
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Description: AFP is Alpha-fetoprotein classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
Name: AFPD,FETA,HPAFP,AFP
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基因ID
- 174
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UniProt
- P02771
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途径
- C21-Steroid Hormone Metabolic Process
抗原
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