产品编号 ABIN7887297
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for NGF 7S (Active) 蛋白 (ABIN7887297)
抗原
NGF 7S
蛋白类型
Native
生物活性
Active
宿主
小鼠
资源
小鼠
应用范围
Cell Culture (CC), Cytotoxicity Test (CyTox)
纯度
≥98 % (HPLC)
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原理
- Native mouse NGF 7S protein
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特异性
- Toxin target: p75 neurotrophin receptor (p75NTR), TrkA receptors
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Biological Activity Comment
- NGF is purified in three forms: the 7S, 2.5S and β. The 7S 130 kDa form occurs naturally in mouse submaxillary glands and is a multimeric protein composed of two α, one β and two γ subunits. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical chains held together by hydrophobic interactions. The α chain stabilizes the 7S complex and binds two zinc ions per 7S complex as a cofactor at the β-γ interface. The γ chain is an arginine specific protease, it may also have plasminongen activator activity as well as mitogenic activity for chick embryo fibroblasts1,2.
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应用备注
- Antigen Preadsorption Control: EC50 = 0.2 nM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain residual acetate.
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注意事项
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture
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储存条件
- -20 °C
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储存方法
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.
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- NGF 7S
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背景
- A native mouse nerve growth factor (NGF) 7S protein complex that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors
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分子量
- 130 kDa
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CAS-编号
- 93928-24-6
抗原
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