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北京 101111
Quick Overview for CNTF 蛋白 (ABIN7491826)
抗原
See all CNTF 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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原理
- Recombinant rat CNTF protein
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序列
- AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
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特异性
- Toxin target: Leukemia Inhibitory Factor (LIF) receptor, gp130, CNTFR
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内毒素水平
- <0.1 EU per 1 μg of the protein by the LAL method.
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Biological Activity Comment
- CNTF is a potent neurotrophic factor that was originally characterized as a survival factor for chick ciliary neurons. CNTF supports growth and survival of DRG, motor and sympathetic neurons1-4.
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Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)
custom-made CNTF 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) (AA 1-198) protein (His tag)custom-made CNTF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (GST tag)CNTF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His-IF2DI Tag)CNTF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 2-198) protein (His-IF2DI Tag)CNTF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)CNTF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) (Active) protein (His tag)CNTF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, AcA Active
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) (Active) proteinCNTF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS, AcA Active
Ciliary Neurotrophic Factor (CNTF) (AA 4-196), (partial) protein (His tag)CNTF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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应用备注
- Antigen Preadsorption Control: EC50 = ~2.2 pM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. For storage of dilute working solutions, we recommend adding a carrier protein (e.g., 0.1 % BSA) to your buffer to prevent material loss due to protein adsorption. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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注意事项
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture
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储存条件
- -20 °C
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储存方法
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.
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- CNTF (Ciliary Neurotrophic Factor (CNTF))
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别名
- CNTF
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背景
- A potent survival factor for neurons and oligodendrocytes
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分子量
- 22.7 kDa
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UniProt
- P20294
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途径
- JAK/STAT Signaling
抗原
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