G Protein-Coupled Receptor 133 Protein (GPR133)
This Synthetic Nanodisc G Protein-Coupled Receptor 133 protein is expressed in HEK-293 Cells.
Quick Overview for G Protein-Coupled Receptor 133 Protein (GPR133) (ABIN7491529)
抗原
See all G Protein-Coupled Receptor 133 (GPR133) 蛋白
G Protein-Coupled Receptor 133 (GPR133)
蛋白类型
Synthetic Nanodisc
宿主
人
资源
HEK-293 Cells
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原理
- Human ADGRD1 full length protein-synthetic nanodisc
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产品特性
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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G Protein-Coupled Receptor 133 (GPR133) (AA 26-570) protein (His tag)
GPR133 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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说明
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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有效期
- 12 months
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- G Protein-Coupled Receptor 133 (GPR133)
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别名
- ADGRD1
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背景
- The adhesion G-protein-coupled receptors (GPCRs), including GPR133, are membrane-bound proteins with long N termini containing multiple domains. GPCRs, or GPRs, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.
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分子量
- The human full length ADGRD1 protein has a MW of 96.5 kDa
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UniProt
- Q6QNK2
抗原
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