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XCR1 Protein (DYKDDDDK Tag)

This Synthetic Nanodisc XCR1 protein is expressed in HEK-293 Cells.
产品编号 ABIN7491763
发货至: 中国
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Quick Overview for XCR1 Protein (DYKDDDDK Tag) (ABIN7491763)

抗原

See all XCR1 蛋白
XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))

蛋白类型

Synthetic Nanodisc

宿主

  • 5
  • 1

资源

  • 2
  • 1
  • 1
  • 1
  • 1
HEK-293 Cells

应用范围

Immunogen (Imm), ELISA, Cryogenic electron microscopy (cryo-EM), Phage Display (PhD), Surface Plasmon Resonance (SPR)
  • 标记

    This XCR1 protein is labelled with DYKDDDDK Tag.

    原理

    Human XCR1 full length protein-synthetic nanodisc

    产品特性

    Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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    Product
    表达系统
    Conjugate
    Origin
    价格从
    表达系统 HEK-293 Cells
    Conjugate His tag
    Origin Human
    价格从 150,642.20 ¥
    表达系统 Cell-free protein synthesis (CFPS)
    Conjugate Strep Tag
    Origin Human
    价格从 145,985.55 ¥

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  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    Advantages:

    • Highly purified membrane proteins
    • High solubility in aqueous solutions
    • High stability
    • Proteins are in a native membrane environment and remain biologically active
    • No detergent and can be used for cell-based assays
    • No MSP backbone proteins
    Limitations:
    • Intolerant to acids and high concentrations of divalent metal ions

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Please see Certificate of Analysis for specific instructions of reconstitution

    缓冲液

    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

    储存条件

    -20 °C,-80 °C

    储存方法

    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

    有效期

    12 months
  • 抗原

    XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))

    别名

    XCR1

    背景

    CCXCR1, GPR5
    The protein is a chemokine receptor belonging to the G protein-coupled receptor superfamily. The family members are characterized by the presence of 7 transmembrane domains and numerous conserved amino acids. This receptor is most closely related to RBS11 and the MIP1-alpha/RANTES receptor. It transduces a signal by increasing the intracellular calcium ions level. The viral macrophage inflammatory protein-II is an antagonist of this receptor and blocks signaling. Several alternatively spliced transcript variants encoding the same protein have been found for this gene.

    分子量

    The human full length XCR1 protein has a MW of 38.5 kDa

    UniProt

    P46094
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