Glucagon Receptor Protein (GCGR)
This Synthetic Nanodisc Glucagon Receptor protein is expressed in HEK-293 Cells.
Quick Overview for Glucagon Receptor Protein (GCGR) (ABIN7491649)
抗原
See all Glucagon Receptor (GCGR) 蛋白
Glucagon Receptor (GCGR)
蛋白类型
Synthetic Nanodisc
宿主
人
资源
HEK-293 Cells
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原理
- Human GCGR full length protein-synthetic nanodisc
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产品特性
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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Glucagon Receptor (GCGR) (AA 26-136) protein (Fc Tag)
GCGR 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Glucagon Receptor (GCGR) (AA 26-136) protein (His tag)GCGR 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
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说明
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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有效期
- 12 months
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- Glucagon Receptor (GCGR)
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别名
- GCGR
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背景
- G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.
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分子量
- The human full length GCGR protein has a MW of 54.0 kDa
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UniProt
- P47871
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途径
- Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
抗原
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