Quick Overview for LGR4 蛋白 (ABIN7491687)
抗原
See all LGR4 蛋白蛋白类型
宿主
资源
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原理
- Human LGR4 full length protein-synthetic nanodisc
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产品特性
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 852-950) protein (GST tag)
LGR4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 25-544) protein (Fc Tag)LGR4 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 25-544) (Active) protein (His tag)LGR4 宿主: 人 宿主: HEK-293 Cells Recombinant >85 % as determined by SDS-PAGE. Active
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说明
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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有效期
- 12 months
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- LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))
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别名
- LGR4
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背景
- Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye.
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分子量
- The human full length LGR4 protein has a MW of 104.5 kDa
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UniProt
- Q9BXB1
抗原
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