IgE Protein (AA 1-432) (His tag)
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Quick Overview for IgE Protein (AA 1-432) (His tag) (ABIN7490715)
抗原
See all IgE 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 1-432
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标记
- This IgE protein is labelled with His tag.
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原理
- Recombinant Cynomolgus IgHE protein with C-terminal 6xHis tag
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特异性
- IgHE(Ala1-Lys432) 6xHis tag
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产品特性
- Extracellular Domain Protein
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纯化方法
- Purified from cell culture supernatant by affinity chromatography
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IgE (AA 209-428) protein (His-Avi Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)宿主: 人 宿主: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-429) protein (His tag)宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-428) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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应用备注
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Extracellular Domain Proteins (ECD) can be used as:
- Immunogens for antibody drug development
- Reagents used for CAR-T positive cell monitoring
- Reagents for antibody screening and functional testing
- Reagents for antibody affinity measurement
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说明
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The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please see Certificate of Analysis for specific instructions of reconstitution.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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有效期
- 12 months
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- IgE
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别名
- IgHE
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背景
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Immunoglobulin heavy constant epsilon,IGHE
IGHE (Immunoglobulin Heavy Constant Epsilon) is a Protein Coding gene. Diseases associated with IGHE include Toxocariasis and Latex Allergy. Among its related pathways are Cytokine Signaling in Immune system and RET signaling. Gene Ontology (GO) annotations related to this gene include antigen binding. An important paralog of this gene is IGHG2. -
分子量
- predicted molecular mass of 48.3 kDa after removal of the signal peptide. The apparent molecular mass of cIgHE-His is 55-70 kDa due to glycosylation.
抗原
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