Nucleolin Protein (NCL) (AA 2-710) (His tag)
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Quick Overview for Nucleolin Protein (NCL) (AA 2-710) (His tag) (ABIN7490643)
抗原
See all Nucleolin (NCL) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 2-710
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标记
- This Nucleolin protein is labelled with His tag.
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原理
- Recombinant human NCL protein with N-terminal 6xHis tag
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特异性
- 6xHis tag NCL(Val2-Glu710)
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产品特性
- Extracellular Domain Protein
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纯化方法
- Purified from cell culture supernatant by affinity chromatography
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Nucleolin (NCL) (AA 1-711) protein (His tag)
NCL 宿主: 猕猴 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Nucleolin (NCL) (AA 2-707) protein (His tag)NCL 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Nucleolin (NCL) protein (Myc-DYKDDDDK Tag)NCL 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Nucleolin (NCL) (AA 2-482), (partial) protein (His tag)NCL 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
Nucleolin (NCL) proteinNCL 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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应用备注
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Extracellular Domain Proteins (ECD) can be used as:
- Immunogens for antibody drug development
- Reagents used for CAR-T positive cell monitoring
- Reagents for antibody screening and functional testing
- Reagents for antibody affinity measurement
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说明
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The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please see Certificate of Analysis for specific instructions of reconstitution.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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有效期
- 12 months
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- Nucleolin (NCL)
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别名
- NCL
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背景
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C23,Nsr1
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008] -
分子量
- predicted molecular mass of 77.3 kDa after removal of the signal peptide. The apparent molecular mass of His-NCL is 100-130 kDa due to glycosylation.
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UniProt
- P19338
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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