HLA-A*03:01 (AA 25-305), (Tetramer) protein (His-Avi Tag,KRAS)
This Recombinant protein is expressed in HEK-293 Cells.
Quick Overview for HLA-A*03:01 (AA 25-305), (Tetramer) protein (His-Avi Tag,KRAS) (ABIN7454302)
抗原
HLA-A*03:01
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
-
-
产品特性
- AA 25-305, Tetramer
-
标记
- His-Avi Tag,KRAS
-
原理
- Human HLA-A*03:01&B2M&KRAS WT (VVVGAGGVGK) Tetramer Protein
-
序列
- Gly25-Thr305(HLA-A*03:01) & Ile21-Met119(B2M)and VVVGAGGVGK peptide
-
特异性
- Uni-Prot: NP_002107.3 (HLA-A*03:01), P61769 (B2M), VVVGAGGVGK
-
产品特性
- Recombinant Human KRAS WT(HLA-A*03:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAGGVGK peptide.
-
过滤
- 0.22 μm filtered
-
内毒素水平
- Less than 1EU per μg by the LAL method.
-
-
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
-
缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
-
储存条件
- -20 °C,-80 °C
-
储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
-
有效期
- 12 months
-
-
- HLA-A*03:01
-
背景
- Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target.
-
分子量
- 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
-
NCBI登录号
- NP_002107
抗原
-
You are here:
