HLA-A*24:02 (AA 25-305), (Monomer) protein (Biotin,His-Avi Tag,GP100 Intron 4)
This Recombinant protein is expressed in HEK-293 Cells.
Quick Overview for HLA-A*24:02 (AA 25-305), (Monomer) protein (Biotin,His-Avi Tag,GP100 Intron 4) (ABIN7454262)
抗原
HLA-A*24:02
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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产品特性
- AA 25-305, Monomer
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标记
- Biotin,His-Avi Tag,GP100 Intron 4
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原理
- Biotinylated Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer Protein
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序列
- Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M)and VYFFLPDHL peptide
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特异性
- Uni-Prot: AAA59600.1 (HLA-A*24:02), P61769 (B2M), VYFFLPDHL
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产品特性
- Recombinant Biotinylated Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M) and VYFFLPDHL peptide.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- HLA-A*24:02
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背景
- Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
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分子量
- 50.40 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Tris-Bis PAGE result.
抗原
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