Fibronectin Protein (AA 32-345) (His tag)
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Quick Overview for Fibronectin Protein (AA 32-345) (His tag) (ABIN7454257)
抗原
See all Fibronectin 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 32-345
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标记
- This Fibronectin protein is labelled with His tag.
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原理
- Human Fibronectin Protein
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序列
- Gln32-Thr345
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产品特性
- Recombinant Human Fibronectin (32-345) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Thr345.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human Fibronectin (32-345) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Fibronectin (32-345) is greater than 95% as determined by SEC-HPLC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Fibronectin
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背景
- Fibronectin is a high molecular glycoprotein present in the blood, connective tissue and at cell surface. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans.
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分子量
- 35.93 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
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UniProt
- P02751
抗原
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