IL-37 Protein (AA 27-192)
This Recombinant IL-37 protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for IL-37 Protein (AA 27-192) (ABIN7454154)
抗原
See all IL-37 (IL37) 蛋白
IL-37 (IL37)
(Interleukin 37 (IL37))
蛋白类型
Recombinant
宿主
人
资源
大肠杆菌（E. Coli）
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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产品特性
- AA 27-192
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原理
- Human IL-37 Protein
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序列
- Lys27-Asp192
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产品特性
- Recombinant Human IL-37 Protein is expressed from E.coli without tag.It contains Lys27-Asp192.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- The affinity constant of 0.12 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 37 (IL37) (AA 1-218) protein (GST tag)
IL37 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 37 (IL37) (Active) proteinIL37 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in 20 mM PBS, 2 mM DTT ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- IL-37 (IL37) (Interleukin 37 (IL37))
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别名
- IL-37
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背景
- IL-37 is a unique member of the IL-1 family of cytokines, which functions as a natural suppressor of inflammatory and immune responses. Immune and non-immune cells produce IL-37 precursor following pro-inflammatory stimuli. Following activating cleavage by caspase-1, mature IL-37 translocates to the nucleus, where it suppresses transcription of pro-inflammatory genes.
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分子量
- 18.55 kDa same as Tris-Bis PAGE result.
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UniProt
- Q9NZH6-2
抗原
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