Cas12a Nuclease (Cpf1) protein (His tag)
This Recombinant Cas12a Nuclease protein is expressed in 大肠杆菌（E. Coli）.
产品编号 ABIN7316792
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Cas12a Nuclease (Cpf1) protein (His tag) (ABIN7316792)
抗原
Cas12a Nuclease (Cpf1)
蛋白类型
Recombinant
宿主
Bacteria (Lachnospiraceae)
资源
大肠杆菌（E. Coli）
纯度
95,00 %
质量等级
MALS verified
-
-
标记
- His tag
-
原理
- GENPower™ NLS-Cas12a Nuclease Protein (MALS verified)
-
品牌
- GENPower™
-
序列
- Ser 2 - His 1228
-
产品特性
- GENPower™ NLS-Cas12a Nuclease is expressed from E.coli cells. It contains AA Ser 2 - His 1228 (Accession # A0A5S8WF58).
-
内毒素水平
- 0.01 EU per μg
-
-
-
-
说明
-
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 147.6 kDa. The protein migrates as 135-145 kDa under reducing (R) condition (SDS-PAGE). This protein has two NLS, which leads to transport to the nucleus efficiently. It is ideal for in vitro and in vivo reactions.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- 20 mM NaAC,500 mM NaCl,0.1 mM EDTA,0.1 mM TCEP, pH 6.0
-
储存条件
- -20 °C
-
储存方法
- -20°C
-
-
- Cas12a Nuclease (Cpf1)
-
别名
- Cas12a Nuclease Protein
-
背景
- Synonyms:CRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1, cas12a, Cpf1,Description:Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated endonuclease from Lachnospiraceae bacterium,Cas12a, also known as Cpf1, is a 1200-1500 amino-acids long monomeric protein that belongs to the CRISPR/Cas system , an adaptive immune system of prokaryotes that has now become a powerful tool for genome editing. CRISPR/Cas12a belongs the class II (type 5) of the CRISPR/Cas system that is defined by a single-subunit effector. Cas12a has recently emerged as an alternative for Cas9, due to its distinct features such as the ability to target T-rich motifs, no need for trans-activating crR, inducing a staggered double-strand break and potential for both R processing and D nuclease activity. In addition, Cas12a is able to process more structured pre-CRISPR/R(crR) molecules into mature crRs which allows the possibility to use both mature or pre-crR for genome editing purposes. All these features make the CRISPR/Cas12a system a valuable genome-engineering tool. CRISPR-Cas12a has been successfully used to edit genomes in many mammalians cells and plants.
-
分子量
- 147.6 kDa
抗原
-
You are here:
