VEGF 165 (AA 27-191) 蛋白
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北京 101111
Quick Overview for VEGF 165 (AA 27-191) 蛋白 (ABIN7275824)
抗原
蛋白类型
宿主
资源
纯度
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产品特性
- AA 27-191
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原理
- Human VEGF165 Protein
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序列
- Ala27-Arg191
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产品特性
- Recombinant Human VEGF165 Protein is expressed from HEK293 without tag.It contains Ala27-Arg191.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 0.05 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 74.2ng/ml determined by ELISA. See testing image for detail.
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VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
VEGF 165 (AA 27-191) protein (His-Avi Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 207-371) protein (His tag)宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
VEGF 165 (AA 207-371) protein宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
VEGF 165 (AA 27-191) protein (His tag)宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 85 % SDS
VEGF 165 protein宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- VEGF 165
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别名
- VEGF165
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背景
- Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.
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分子量
- 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result.
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UniProt
- P15692-4
抗原
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