TNC Protein (AA 23-621) (His tag)
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Quick Overview for TNC Protein (AA 23-621) (His tag) (ABIN7275753)
抗原
See all TNC 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 23-621
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标记
- This TNC protein is labelled with His tag.
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原理
- Human Tenascin Protein
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序列
- Gly23-Ser621
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产品特性
- Recombinant Human Tenascin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly23-Ser621.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human Tenascin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Tenascin is greater than 95% as determined by SEC-HPLC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- TNC (Tenascin C (TNC))
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别名
- Tenascin
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背景
- Tenascin-C (TNC) is a hexameric, multimodular extracellular matrix protein with several molecular forms that are created through alternative splicing and protein modifications. It is highly conserved amongst vertebrates, and molecular phylogeny indicates that it evolved before fibronectin. Tenascin-C has many extracellular binding partners, including matrix components, soluble factors and pathogens, it also influences cell phenotype directly through interactions with cell surface receptors.
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分子量
- 65.27 kDa. Due to glycosylation, the protein migrates to 66-70 kDa based on Bis-Tris PAGE result.
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UniProt
- P24821
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途径
- Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
抗原
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