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SEMA4D/CD100 Protein (AA 22-734) (His-Avi Tag)

Name: SEMA4D/CD100 Protein (AA 22-734) (His-Avi Tag)
Brand: antibodies-online
SKU: ABIN7275565
Availability: OutOfStock

This Recombinant SEMA4D/CD100 protein is expressed in HEK-293 Cells.

Human Semaphorin 4D on Tris-Bis PAGE under reduced condition. (SEMA4D/CD100 Protein (AA 22-734) (His-Avi Tag))

SDS

SEC-HPLC

Immobilized Human Semaphorin 4D, His Tag at 1 μg/mL (100 μL/well) on the plate. (SEMA4D/CD100 Protein (AA 22-734) (His-Avi Tag))

ELISA

3 images

产品编号 ABIN7275565

发货至: 中国

Quick Overview for SEMA4D/CD100 Protein (AA 22-734) (His-Avi Tag) (ABIN7275565)

抗原

See all SEMA4D/CD100 (SEMA4D) 蛋白

SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

蛋白类型

Recombinant

宿主

人

资源

HEK-293 Cells

纯度

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Discover our top product SEMA4D 蛋白

产品特性

AA 22-734

标记

This SEMA4D/CD100 protein is labelled with His-Avi Tag.

原理

Human Semaphorin 4D/SEMA4D/CD100 Protein

序列

Met22-Arg734

产品特性

Recombinant Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Arg734.

过滤

0.22 μm filtered

内毒素水平

Less than 1EU per μg by the LAL method.

Biological Activity Comment

Immobilized Human Semaphorin 4D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
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Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 22-734) protein (His-Avi Tag,Biotin)
SEMA4D 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 24-734) protein (His tag)
SEMA4D 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 24-733) protein (His tag)
SEMA4D 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) protein (Fc Tag)
SEMA4D 宿主: 小鼠宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 759-862) protein (His-IF2DI Tag)
SEMA4D 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) protein (Fc Tag)
SEMA4D 宿主: 大鼠宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (Active) protein (His tag)
SEMA4D 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) protein (His tag)
SEMA4D 宿主: 大鼠宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 22-734) protein (His tag)
SEMA4D 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (Active) protein (Fc Tag)
SEMA4D 宿主: 人宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE. Active

限制

仅限研究用
状态

Lyophilized

溶解方式

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

缓冲液

Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

储存条件

-20 °C,-80 °C

储存方法

-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

有效期

12 months
抗原

SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

别名

Semaphorin 4D

背景

Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.

分子量

82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.

UniProt

Q92854-1

途径

Regulation of Cell Size