MMP8 Protein (AA 21-467) (His tag)
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北京 101111
Quick Overview for MMP8 Protein (AA 21-467) (His tag) (ABIN7275269)
抗原
See all MMP8 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 21-467
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标记
- This MMP8 protein is labelled with His tag.
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原理
- Human MMP-8 Protein (active form)
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序列
- Phe21-Gly467
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产品特性
- Recombinant Human MMP-8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe21-Gly467.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 uM as determined in SPR assay (Biacore T200).,Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 4.66ug/ml determined by ELISA (QC Test).,Human MMP-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 300 pmoles/min/ug (QC Test).,The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.
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Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-467) (Active) protein (His tag)
MMP8 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-465) (Active) protein (His tag)MMP8 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) proteinMMP8 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 1-467) protein (GST tag)MMP8 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-467) protein (His tag)MMP8 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
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别名
- MMP-8
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背景
- Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.
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分子量
- 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.
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UniProt
- P22894
抗原
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