IgG1 Fc (AA 98-324) (Active) 蛋白
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北京 101111
Quick Overview for IgG1 Fc (AA 98-324) (Active) 蛋白 (ABIN7275250)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 98-324
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原理
- Mouse IgG1 Fc Protein
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序列
- Val98-Lys324
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产品特性
- Recombinant Mouse IgG1 Fc Protein is expressed from HEK293 without tag.It contains Val98-Lys324.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200).,Mouse IgG1 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse IgG1 Fc is greater than 90% as determined by SEC-HPLC.
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IgG1 Fc (AA 104-330) (Active) protein
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgG1 Fc protein (Fc Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
IgG1 Fc protein宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. WB
IgG1 Fc (AA 100-330) protein (gD Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- IgG1 Fc
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背景
- Recombinant murine Fc fragment contains the hinge region, CH2, and CH3 region of the mouse IgG1 isotype. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.
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分子量
- 25.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
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UniProt
- P01868
抗原
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