IL27 Receptor alpha Protein (AA 34-516) (hFc Tag)
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Quick Overview for IL27 Receptor alpha Protein (AA 34-516) (hFc Tag) (ABIN7275016)
抗原
See all IL27 Receptor alpha (IL27RA) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 34-516
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标记
- This IL27 Receptor alpha protein is labelled with hFc Tag.
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原理
- Human IL-27RA/TCCR Protein
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序列
- Gly34-Lys516
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产品特性
- Recombinant Human IL-27RA/TCCR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly34-Lys516.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA (QC Test).,Human IL-27RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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Interleukin 27 Receptor, alpha (IL27RA) (AA 34-516) protein (His tag)
IL27RA 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Interleukin 27 Receptor, alpha (IL27RA) (AA 33-132) protein (GST tag)IL27RA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 27 Receptor, alpha (IL27RA) (AA 33-514) protein (Fc Tag)IL27RA 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- IL27 Receptor alpha (IL27RA) (Interleukin 27 Receptor, alpha (IL27RA))
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别名
- IL-27 Ra
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背景
- A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, http://tccr.unmc.edu) allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects.
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分子量
- 79.3 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
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UniProt
- Q6UWB1
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途径
- Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response
抗原
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