IL-10 Protein (AA 19-178)
Quick Overview for IL-10 Protein (AA 19-178) (ABIN7274884)
抗原
See all IL-10 (IL10) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 19-178
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原理
- Human IL-10 Protein
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序列
- Ser19-Asn178
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产品特性
- Recombinant Human IL-10 Protein is expressed from HEK293 without tag.It contains Ser19-Asn178.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 0.1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 47.4ng/ml determined by ELISA. The affinity constant of 0.255 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 10 (IL10) (AA 19-178) protein (His-Avi Tag)
IL10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 10 (IL10) (AA 19-178) protein (His-Avi Tag,Biotin)IL10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 10 (IL10) (AA 19-178) (Active) proteinIL10 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 10 (IL10) (Active) proteinIL10 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 10 (IL10) (AA 22-178) protein (His tag)IL10 宿主: Hamster 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 10 (IL10) (AA 1-178) protein (GST tag)IL10 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 10 (IL10) proteinIL10 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Interleukin 10 (IL10) (AA 19-178) protein (His tag)IL10 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- IL-10 (IL10) (Interleukin 10 (IL10))
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别名
- IL-10
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背景
- Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.
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分子量
- 18.8 kDa same as Tris-Bis PAGE result.
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UniProt
- P22301
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
抗原
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