IL-18 Protein (AA 37-193)
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- 抗原 See all IL-18 (IL18) 蛋白
- IL-18 (IL18) (Interleukin 18 (IL18))
- 蛋白类型
- Recombinant
- 产品特性
- AA 37-193
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 原理
- Human IL-18 Protein
- 序列
- Tyr37-Asp193
- 产品特性
- Recombinant Human IL-18 Protein is expressed from E.coli without tag.It contains Tyr37-Asp193.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 0.02 EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-18 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA. The affinity constant of 1.21nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 18 (IL18) (AA 37-193) protein (Biotin)
IL18 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Interleukin 18 (IL18) (AA 36-192) protein (His tag)IL18 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Interleukin 18 (IL18) (AA 37-193) protein (His-IF2DI Tag)IL18 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 18 (IL18) (AA 1-193) protein (GST tag)IL18 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 18 (IL18) protein (GST tag)IL18 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % as determined by reducing SDS-PAGE.
Interleukin 18 (IL18) protein (His tag)IL18 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Interleukin 18 (IL18) (AA 37-197) protein (Fc Tag)IL18 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 18 (IL18) (AA 37-197) protein (mFc Tag)IL18 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in 20 mM PB, 250 mM NaCl, 50 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- IL-18 (IL18) (Interleukin 18 (IL18))
- 别名
- IL-18 (IL18 产品)
- 背景
- Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naive T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
- 分子量
- 18.2 kDa same as Tris-Bis PAGE result.
- UniProt
- Q14116
- 途径
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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