IgG2a Fc (AA 98-330) 蛋白
This Recombinant protein is expressed in HEK-293 Cells.
Quick Overview for IgG2a Fc (AA 98-330) 蛋白 (ABIN7274869)
抗原
IgG2a Fc
蛋白类型
Recombinant
宿主
小鼠
资源
HEK-293 Cells
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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产品特性
- AA 98-330
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原理
- Mouse IgG2a Fc Protein
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序列
- Glu98-Lys330
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产品特性
- Recombinant Mouse IgG2a Fc Protein is expressed from HEK293 without tag.It contains Glu98-Lys330.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- The affinity constant of 2.72 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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IgG2a Fc protein (Fc Tag)
宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- IgG2a Fc
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背景
- Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life.
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分子量
- 26.38 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Tris-Bis PAGE result.
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UniProt
- P01863
抗原
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