IgG2a Fc (AA 98-330) (Active) 蛋白
Our Local Distributor
北京 101111
Quick Overview for IgG2a Fc (AA 98-330) (Active) 蛋白 (ABIN7274869)
抗原
蛋白类型
生物活性
宿主
资源
纯度
-
-
产品特性
- AA 98-330
-
原理
- Mouse IgG2a Fc Protein
-
序列
- Glu98-Lys330
-
产品特性
- Recombinant Mouse IgG2a Fc Protein is expressed from HEK293 without tag.It contains Glu98-Lys330.
-
过滤
- 0.22 μm filtered
-
内毒素水平
- Less than 1 EU per μg by the LAL method.
-
Biological Activity Comment
- Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).,Mouse IgG2a Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse IgG2a Fc is greater than 95% as determined by SEC-HPLC.
-
-
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
-
缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
-
储存条件
- -20 °C,-80 °C
-
储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
-
有效期
- 12 months
-
-
- IgG2a Fc
-
背景
- Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life.
-
分子量
- 26.38 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Bis-Tris PAGE result.
-
UniProt
- P01863
抗原
-