IgG1 Fc (AA 104-330) (Active) 蛋白
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北京 101111
Quick Overview for IgG1 Fc (AA 104-330) (Active) 蛋白 (ABIN7274867)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 104-330
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原理
- Human IgG1 Fc Protein
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序列
- Asp104-Lys330
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产品特性
- Recombinant Human IgG1 Fc is expressed from HEK293 without tag.It contains Asp104-Lys330.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).,Human IgG1 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Biotinylated Human FcRn, His-Avi Tag on Streptavidin-Biosensor can bind Human IgG1 Fc, No Tag with an affinity constant of 22.8 nM as determined in BLI assay (Gator® Prime).,The purity of Human IgG1 Fc is greater than 95% as determined by SEC-HPLC.
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IgG1 Fc (AA 98-324) (Active) protein
宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
IgG1 Fc protein (Fc Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
IgG1 Fc protein宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. WB
IgG1 Fc (AA 100-330) protein (gD Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- IgG1 Fc
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背景
- IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
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分子量
- 25.5 kDa. Due to glycosylation, the protein migrates to 31-33 kDa based on Bis-Tris PAGE result.
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UniProt
- P01857
抗原
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