Hepsin Protein (HPN) (AA 45-417) (His tag)
This Recombinant Hepsin protein is expressed in HEK-293 Cells.
Quick Overview for Hepsin Protein (HPN) (AA 45-417) (His tag) (ABIN7274824)
抗原
See all Hepsin (HPN) 蛋白
Hepsin (HPN)
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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产品特性
- AA 45-417
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标记
- This Hepsin protein is labelled with His tag.
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原理
- Human HPN Protein
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序列
- Arg45-Leu417
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产品特性
- Recombinant Human HPN Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg45-Leu417.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human HPN, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 22.8ng/ml determined by ELISA. See testing image for detail.
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Hepsin (HPN) (AA 60-436) protein (His tag)
HPN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Hepsin (HPN) (AA 40-417) protein (GST tag)HPN 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM NaAc,150 mM NaCl ( pH 5.0).
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缓冲液
- Lyophilized from 0.22μm filtered solution in 20 mM NaAc,150 mM NaCl ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Hepsin (HPN)
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别名
- HPN
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背景
- Home parenteral nutrition (HPN) has been proposed as the treatment of choice in patients suffering from intestinal failure (IF) and has been claimed to improve survival and quality of life either in patients with benign disorders or even in those with malignancies. HPN seems to have beneficial effect but it should be considered with caution by the physicians who should take into account the indications of each patient to receive parenteral nutrition, the underlying disease and prognosis and the access of each patient to home care services.
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分子量
- 42 kDa. Due to the protein cleaved into the following two chains, so it migrates to 18-22 kDa and 30 kDa based on Tris-Bis PAGE result.
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UniProt
- P05981
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途径
- Regulation of Hormone Metabolic Process, Sensory Perception of Sound
抗原
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