TNFSF18 Protein (AA 50-177, Trimer) (Fc Tag)
Our Local Distributor
北京 101111
Quick Overview for TNFSF18 Protein (AA 50-177, Trimer) (Fc Tag) (ABIN7274679)
抗原
See all TNFSF18 蛋白蛋白类型
生物活性
宿主
资源
纯度
-
-
产品特性
- AA 50-177, Trimer
-
标记
- This TNFSF18 protein is labelled with Fc Tag.
-
原理
- Human GITR Ligand/TNFSF18 Trimer Protein
-
序列
- Gln50-Ser177
-
产品特性
- Recombinant Human GITR Ligand/TNFSF18 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Gln50-Ser177 trimer design.
-
过滤
- 0.22 μm filtered
-
内毒素水平
- Less than 1 EU per μg by the LAL method.
-
Biological Activity Comment
- Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).,Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test).,Human GITR Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human GITR Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.
-
-
Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18) (AA 50-177), (Trimer) (Active) protein (Biotin,His-DYKDDDDK Tag)
TNFSF18 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18) (AA 50-177), (Trimer) (Active) protein (His-DYKDDDDK Tag)TNFSF18 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18) (AA 72-199) (Active) protein (His tag)TNFSF18 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18) (AA 1-173) protein (His tag)custom-made TNFSF18 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18) (Active) proteinTNFSF18 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18) (AA 47-173) (Active) protein (hFc Tag)TNFSF18 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18) (AA 99-330) protein (His tag,mFc Tag)TNFSF18 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18) (AA 50-177) protein (His tag)TNFSF18 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18) (AA 50-177) protein (His-DYKDDDDK-Strep II Tag)TNFSF18 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18) (AA 50-177) (Active) protein (His tag)TNFSF18 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
-
缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
-
储存条件
- -20 °C,-80 °C
-
储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
-
有效期
- 12 months
-
-
- TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))
-
别名
- GITR Ligand
-
背景
- Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.
-
分子量
- 70.2 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result.
-
UniProt
- Q9UNG2
抗原
-
