FCGR1 Protein (AA 16-288) (His-Avi Tag)
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Quick Overview for FCGR1 Protein (AA 16-288) (His-Avi Tag) (ABIN7274672)
抗原
See all FCGR1 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 16-288
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标记
- This FCGR1 protein is labelled with His-Avi Tag.
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原理
- Non-biotinylated Human Fc gamma RI/CD64 Protein
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序列
- Gln16-Pro288
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产品特性
- Recombinant Human Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln16-Pro288.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human Fc gamma RI, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 5.25 nM as determined in SPR assay (Biacore T200).,Human Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human Fc gamma RI, His-Avi Tag on Anti-His-Biosensor, can bind Trastuzumab with an affinity constant of 11.70 nM as determined in BLI assay (Gator).
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Fc Receptor, IgG, High Affinity I (FCGR1) (AA 16-288) (Active) protein (Biotin,His-Avi Tag)
FCGR1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Fc Receptor, IgG, High Affinity I (FCGR1) protein (His tag)FCGR1 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Fc Receptor, IgG, High Affinity I (FCGR1) (Active) protein (His tag)FCGR1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Fc Receptor, IgG, High Affinity I (FCGR1) (Active) protein (Biotin,His-Avi Tag)FCGR1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Fc Receptor, IgG, High Affinity I (FCGR1) (AA 73-294) protein (His tag,T7 tag)FCGR1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % IP, SDS, ELISA, WB
Fc Receptor, IgG, High Affinity I (FCGR1) (AA 35-235) protein (His tag,T7 tag)FCGR1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % IP, SDS, ELISA, WB
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))
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别名
- Fc gamma RI
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背景
- The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains, these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
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分子量
- 33.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
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UniProt
- P12314
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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