DKK3 Protein (AA 23-350) (His-Avi Tag)
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Quick Overview for DKK3 Protein (AA 23-350) (His-Avi Tag) (ABIN7274470)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 23-350
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标记
- This DKK3 protein is labelled with His-Avi Tag.
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原理
- Non-biotinylated Human DKK3 Protein
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序列
- Pro23-Ile350
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产品特性
- Recombinant Human DKK3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro23-Ile350.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human DKK3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Human DKK3 is greater than 95% as determined by SEC-HPLC.
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DKK3 (AA 23-350) (Active) protein (Biotin,His-Avi Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
DKK3 (AA 1-350) protein (GST tag)宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
DKK3 (AA 22-350) (Active) protein (His tag)宿主: 人 宿主: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
DKK3 (AA 22-350) protein (His tag)宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- DKK3
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背景
- Dkk-3, also known as REIC (Reduced Expansion in Immortalized Cells), is one of four numbered members of the Dickkopf family of Wnt antagonists.DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
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分子量
- 39.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
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UniProt
- Q9UBP4
抗原
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