CD79b Protein (AA 29-159) (Biotin,His-Avi Tag)
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Quick Overview for CD79b Protein (AA 29-159) (Biotin,His-Avi Tag) (ABIN7274235)
抗原
See all CD79b (CD79B) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 29-159
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标记
- This CD79b protein is labelled with Biotin,His-Avi Tag.
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原理
- Biotinylated Human CD79B Protein
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序列
- Ala29-Asp159
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产品特性
- Recombinant Biotinylated Human CD79B Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, It contains Ala29-Asp159.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Anti-CD79B Antibody, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human CD79B, His Tag with the EC50 of 23.4 ng/ml determined by ELISA.,Biotinylated CD79B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated CD79B is greater than 95% as determined by SEC-HPLC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 24 months
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- CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))
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别名
- CD79B
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背景
- CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta-chain) is a 36-40 kDa member of the Ig-Superfamily. It is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
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分子量
- 18.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
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UniProt
- P40259
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途径
- BCR Signaling
抗原
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