CD79b Protein (AA 29-159) (His-Avi Tag,Biotin)
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- 抗原 See all CD79b (CD79B) 蛋白
- CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))
- 蛋白类型
- Recombinant
- 产品特性
- AA 29-159
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD79b protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human CD79B Protein
- 序列
- Ala29-Asp159
- 产品特性
- Recombinant Biotinylated Human CD79B Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala29-Asp159.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-CD79B Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD79B, His Tag with the EC50 of 23.3ng/ml determined by ELISA. See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))
- 别名
- CD79B (CD79B 产品)
- 背景
- CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta-chain) is a 36-40 kDa member of the Ig-Superfamily. It is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
- 分子量
- 18.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
- UniProt
- P40259-1
- 途径
- BCR Signaling
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