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BMP2 Protein (AA 283-396)

This Recombinant BMP2 protein is expressed in 大肠杆菌(E. Coli).
产品编号 ABIN7273975
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for BMP2 Protein (AA 283-396) (ABIN7273975)

抗原

See all BMP2 蛋白
BMP2 (Bone Morphogenetic Protein 2 (BMP2))

蛋白类型

Recombinant

生物活性

Active

宿主

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人, 犬, 大鼠, 小鼠, 猕猴

资源

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大肠杆菌(E. Coli)

纯度

> 95 % as determined by Bis-Tris PAGE
  • 产品特性

    AA 283-396

    原理

    Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein

    序列

    Gln283-Arg396 (Human/Rhesus macaque) / Gln279-Arg393 (Mouse/Rat) / Gln282-Arg395 (Canine)

    产品特性

    Recombinant Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein is expressed from E.coli without tag.It contains Gln283-Arg396 (Human/Rhesus macaque) / Gln279-Arg393 (Mouse/Rat) / Gln282-Arg395 (Canine).

    过滤

    0.22 μm filtered

    内毒素水平

    Less than 0.5 EU per μg by the LAL method.

    Biological Activity Comment

    Human/Rhesus macaque/Mouse/Rat/Canine BMP2 on Bis-Tris PAGE under Non reduced (N) condition and reduced condition. The purity is greater than 95%.,Measured by its ability to induce Alkaline Phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is 40-200 ng/mL (QC Test).
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  • 限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Dissolve the lyophilized protein in 100 mM Acetic Acid ( pH 2.8). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

    缓冲液

    Lyophilized from 0.22μm filtered solution in 100 mM Acetic Acid ( pH 2.8). Normally 8 % trehalose is added as protectant before lyophilization.

    储存条件

    -20 °C,-80 °C

    储存方法

    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    BMP2 (Bone Morphogenetic Protein 2 (BMP2))

    别名

    BMP2

    背景

    Bone morphogenetic protein 2 (BMP2), a member of the transforming growth factor-β (TGF-β) super-family, is one of the main chondrogenic growth factors involved in cartilage regeneration. BMP2 is known to induce chondrogenic differentiation in various types of stem cells in vitro. However, BMP2 also induces osteogenic differentiation and endochondral ossification in mesenchymal stem cells (MSCs).

    分子量

    12.90 kDa same as Bis-Tris PAGE result.

    UniProt

    P12643

    途径

    Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation
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