APOE Protein (AA 19-317) (Fc Tag)
Quick Overview for APOE Protein (AA 19-317) (Fc Tag) (ABIN7273868)
抗原
See all APOE 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 19-317
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标记
- This APOE protein is labelled with Fc Tag.
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原理
- Human APOE4/Apolipoprotein E Protein
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序列
- Lys19-His317
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产品特性
- Recombinant Human APOE4/Apolipoprotein E Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Lys19-His317.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- The affinity constant of 11.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Apolipoprotein E (APOE) (AA 19-311) protein (Fc Tag)
APOE 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Apolipoprotein E (APOE) (AA 19-311) protein (His tag)APOE 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Apolipoprotein E (APOE) (AA 19-311) protein (His tag)APOE 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Apolipoprotein E (APOE) protein (Myc-DYKDDDDK Tag)APOE 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS, 2 mM DTT, 5 mM CHAPS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- APOE (Apolipoprotein E (APOE))
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别名
- APOE4
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背景
- Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).
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分子量
- 61.6 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result.
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途径
- Regulation of Cell Size, Lipid Metabolism
抗原
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