ApoE3 Protein (AA 19-317) (His tag)
This Recombinant ApoE3 protein is expressed in HEK-293 Cells.
产品编号 ABIN7273865
发货至:
中国
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Contact Info
Our Local Distributor
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Quick Overview for ApoE3 Protein (AA 19-317) (His tag) (ABIN7273865)
抗原
ApoE3
(Apolipoprotein E3 (ApoE3))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
HEK-293 Cells
纯度
> 95 % as determined by Bis-Tris PAGE,> 85 % as determined by HPLC
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产品特性
- AA 19-317
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标记
- This ApoE3 protein is labelled with His tag.
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原理
- Human APOE3/Apolipoprotein E Protein
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序列
- Lys19-His317
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产品特性
- Recombinant Human APOE3/Apolipoprotein E Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Lys19-His317.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human APOE3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human APOE3 is greater than 85% as determined by SEC-HPLC.
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Apolipoprotein E3 (ApoE3) (AA 19-317) (Active) protein (Biotin,His tag)
ApoE3 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- ApoE3 (Apolipoprotein E3 (ApoE3))
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别名
- APOE3
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背景
- Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).
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分子量
- 35.3 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
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UniProt
- P02649
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途径
- Regulation of Cell Size, Lipid Metabolism
抗原
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