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CHIA Protein (AA 22-473) (His tag)

This Recombinant CHIA protein is expressed in HEK-293 Cells.

Mouse AMCase/CHIA on Tris-Bis PAGE under reduced condition. (CHIA Protein (AA 22-473) (His tag))

The purity of Mouse AMCase/CHIA is greater than 95 % as determined by SEC-HPLC. (CHIA Protein (AA 22-473) (His tag))

2 images

产品编号 ABIN7273825

发货至: 中国

Quick Overview for CHIA Protein (AA 22-473) (His tag) (ABIN7273825)

抗原

See all CHIA 蛋白

CHIA (Chitinase, Acidic (CHIA))

蛋白类型

Recombinant

宿主

小鼠

资源

HEK-293 Cells

纯度

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

产品特性

AA 22-473

标记

This CHIA protein is labelled with His tag.

原理

Mouse AMCase/CHIA Protein

序列

Tyr22-Pro473

产品特性

Recombinant Mouse AMCase/CHIA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Pro473.

过滤

0.22 μm filtered

内毒素水平

Less than 1EU per μg by the LAL method.
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Chitinase, Acidic (CHIA) (AA 22-476) protein (His tag)
CHIA 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Chitinase, Acidic (CHIA) (AA 1-368) protein (GST tag)
CHIA 宿主: 人宿主: 小麦胚 Recombinant ELISA, AA, AP, WB

Chitinase, Acidic (CHIA) protein (His tag)
CHIA 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD

Chitinase, Acidic (CHIA) (AA 22-472) protein (His tag)
CHIA 宿主: Cow 宿主: 酵母菌 Recombinant > 90 % ELISA

Chitinase, Acidic (CHIA) (AA 22-473) protein (His tag)
CHIA 宿主: 大鼠宿主: 酵母菌 Recombinant > 90 % ELISA

限制

仅限研究用
状态

Lyophilized

溶解方式

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

缓冲液

Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

储存条件

-20 °C,-80 °C

储存方法

-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

有效期

12 months
抗原

CHIA (Chitinase, Acidic (CHIA))

别名

AMCase

背景

Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. The expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation.

分子量

51 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.

UniProt

Q91XA9

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