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Quick Overview for ACVA Protein (AA 311-426) (ABIN7273796)
抗原
See all ACVA 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 311-426
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原理
- Human/Mouse/Rat Activin A Protein
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序列
- Gly311-Ser426
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产品特性
- Recombinant Human/Mouse/Rat Activin A Protein is expressed from HEK293 without tag.It contains Gly311-Ser426.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 0.1 EU per μg by the LAL method.
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Biological Activity Comment
- Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human/Mouse/Rat Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).,Human ACVR1B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 18.43 nM as determined in SPR assay (Biacore T200).,Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test).,Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.,Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.,Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test).
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Activin A (ACVA) (AA 311-426) (Active) protein
ACVA 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Activin A (ACVA) (AA 21-426) (Active) protein (His tag)ACVA 宿主: 人 宿主: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in 4 mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in 4 mM HCI. Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- ACVA (Activin A (ACVA))
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别名
- Activin A
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背景
- Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.
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分子量
- 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
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UniProt
- P08476
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途径
- Hormone Transport, Peptide Hormone Metabolism
抗原
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