Nuclease 蛋白
This Recombinant Nuclease protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for Nuclease 蛋白 (ABIN7271658)
抗原
Nuclease
蛋白类型
Recombinant
宿主
Serratia marcescens
资源
大肠杆菌（E. Coli）
纯度
>95 % as determined by SDS-PAGE.
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原理
- GENIUS™ Nuclease, premium grade
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序列
- Met 1 - Asn 266
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产品特性
- GENIUS™ Pro Nuclease is a tag free recombinant form of Serratia marcescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™ Pro Nuclease is a homodimer with monomer molecular masses about 30 kDa with an isoelectric point (pI) at pH 6.85. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'- phosphorylated oligonucleotides of 3-8 bases in length.
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内毒素水平
- Less than 0.01 EU per μg by the LAL method.
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应用备注
- This protein carries no "tag".
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 20 mM Tris, 20 mM NaCl, 2 mM MgCl2, pH 8.0
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储存条件
- -80 °C
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储存方法
- -70°C
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- Nuclease
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背景
- Synonyms:GENIUS™Nuclease,BenzNuclease,BenzoNuclease,benzonase,Nuclease,Description:NA
抗原
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