IgG2a Fc protein (Fc Tag)
This Recombinant protein is expressed in Mammalian Cells.
Quick Overview for IgG2a Fc protein (Fc Tag) (ABIN7211747)
抗原
IgG2a Fc
蛋白类型
Recombinant
宿主
小鼠
资源
Mammalian Cells
应用范围
In vivo Studies (in vivo), Isotype Control (IsoC)
纯度
> 95 %
质量等级
In vivo Grade
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标记
- Fc Tag
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原理
- In Vivo Grade Recombinant Mouse IgG2a Fc Protein
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产品特性
- The in vivo grade recombinant mouse IgG2a Fc protein (mIgG2a Fc, Glu 98 - Lys 330 of the constant region of the IgG2a heavy chain, Accession # CAC20702) was produced in mammalian cells and purified by protein A. The mIgG2a Fc protein includes the CH2 (123-220) and CH3 (236-318) domains of the heavy chain constant region as well as the hinge region (98-122) before the CH2 domain.
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纯化方法
- Protein A
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过滤
- Sterile filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg)
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IgG2a Fc (AA 98-330) protein
宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgG2a Fc protein (Fc Tag)宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
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应用备注
- The recombinant mouse IgG2a Fc protein and mutants can be used as the isotype control of the mIgG2a antibodies and the mIgG2a Fc fusion proteins in various in vitro and in vivo experiments.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH 7.4
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储存条件
- -20 °C
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储存方法
- 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
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有效期
- 3 months
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- IgG2a Fc
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分子量
- Predicted 26.4 kDa. Due to glycosylation, the recombinant mouse IgG2a Fc protein migrates to 30-35 kDa based on the Bis-Tris PAGE result.
抗原
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