BTK Kinase (Active) protein (His tag)
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北京 101111
Quick Overview for BTK Kinase (Active) protein (His tag) (ABIN7317103)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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标记
- His tag
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原理
- Recombinant Human Bruton Tyrosine Kinase/BTK Kinase Protein (His Tag)(Active)
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序列
- Met 1-Ser 659
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产品特性
- A DNA sequence encoding the human BTK (NP_000052.1) (Met 1-Ser 659) was expressed with a C-terminal polyhistidine tag.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- 1. The specific activity was determined to be 115 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate.2. Measured by its ability to bind human BLNK in a functional ELISA.
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限制
- 仅限研究用
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状态
- Frozen, Liquid
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缓冲液
- Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.0
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- BTK Kinase
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背景
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Background: Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Synonym: AGMX1;AT;ATK;BPK;IMD1;PSCTK1;XLA
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分子量
- 77.8 kDa
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NCBI登录号
- NP_000052
抗原
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