AMPK (A2/B1/G1) (Active) 蛋白
This Recombinant protein is expressed in Baculovirus infected Insect Cells.
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Quick Overview for AMPK (A2/B1/G1) (Active) 蛋白 (ABIN7317039)
抗原
AMPK (A2/B1/G1)
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
Baculovirus infected Insect Cells
纯度
> 94 % as determined by reducing SDS-PAGE.
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原理
- Recombinant Human AMPK (G1/B1/A2) Heterotrimer Protein (Active)
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序列
- Met 1-Pro 331
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产品特性
- A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1, A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2, A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
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说明
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40, 42 & 95 kDa
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限制
- 仅限研究用
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状态
- Frozen, Liquid
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缓冲液
- Supplied as sterile 50 mM Tris, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.5 mM PMSF, 10 % gly, 1 mM GSH, pH 7.4
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- AMPK (A2/B1/G1)
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背景
- AMPK,AMPKa1
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分子量
- 158 (38+30+90) kDa
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UniProt
- P54619
抗原
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