Tryptophan Synthase alpha Chain/Trp A 蛋白
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- 抗原
- Tryptophan Synthase alpha Chain/Trp A
- 蛋白类型
- Recombinant
- 宿主
- E. coli
- 资源
- 大肠杆菌（E. Coli）
- 原理
- Recombinant E.coli Tryptophan Synthase α Chain/Trp A Protein
- 序列
- Met1-Ser268
- 产品特性
- Recombinant E.coli Tryptophan synthase alpha chain is produced by our E.coli expression system and the target gene encoding Met1-Ser268 is expressed.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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- 说明
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29 kDa
- 限制
- 仅限研究用
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- 状态
- Frozen, Liquid
- 缓冲液
- Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C
- 储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- 抗原
- Tryptophan Synthase alpha Chain/Trp A
- 背景
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Background: Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation.
Synonym: Tryptophan synthase alpha chain, trpA,
- 分子量
- 28.7 kDa
- UniProt
- P0A877
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