Quick Overview for SIGLEC10 Protein (Biotin) (ABIN7319878)
抗原
See all SIGLEC10 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This SIGLEC10 protein is labelled with Biotin.
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原理
- Recombinant Human Siglec-10 (C-Fc-Avi) Biotinylated
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序列
- Met17--Thr546
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产品特性
- Biotinylated Recombinant Human Sialic Acid-binding Ig-like Lectin 10 is produced by our Mammalian expression system and the target gene encoding Met17-Thr546 is expressed with a Fc, Avi tag at the C-terminus.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Immobilized Anti-Human Siglec10 mAb(Cat#NC035) at 1μg/ml (100 μl/well) can bind Biotinylated Human Siglec-10-Fc-Avi(Cat#CY47 ). The ED50 of Biotinylated Human Siglec-10-Fc-Avi(Cat#CY47 ) is 1.45 ng/ml.
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Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (AA 17-546) protein (His tag)
SIGLEC10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (AA 17-546) protein (His-Avi Tag,Biotin)SIGLEC10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (AA 17-546), (R119A) protein (His-Avi Tag,Biotin)SIGLEC10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (AA 17-546) protein (Fc-Avi Tag,Biotin)SIGLEC10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (AA 17-546), (R119A) protein (His-Avi Tag)SIGLEC10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (AA 17-546) protein (Fc-Avi Tag)SIGLEC10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (AA 17-552) protein (His tag)SIGLEC10 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (Active) protein (mFc Tag)SIGLEC10 宿主: 人 宿主: 人细胞 Recombinant >90 % as determined by reducing SDS-PAGE. Active
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (Active) protein (Fc Tag)SIGLEC10 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) protein (Fc Tag)SIGLEC10 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
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说明
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100-130 kDa
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))
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别名
- Siglec-10
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背景
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Background: Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.
Synonym: SIGLEC10, MGC126774, PRO940, Siglec10, SLG2, sialic acid-binding Ig-like lectin 10, Siglec-10, siglec-like gene 2, Siglec-like protein 2, SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7
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分子量
- 86.9 kDa
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UniProt
- Q96LC7
抗原
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